S4697
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 | Immunoblotting Total cell extract of HEK-293T cells transfected with human Seladin-1 expression vector was separated on SDS-PAGE and probed with Monoclonal Anti-Seladin-1 (Cat. No. S4697). The antibody was developed with Anti-Mouse IgG, Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1: 2 μg/mL antibody 2: 1 μg/mL antibody 3: 0.5 μg/mL antibody |
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S4697
Sigma
Monoclonal Anti-Seladin-1 antibody produced in mouse
~2 mg/mL, clone SL-14, purified immunoglobulin, buffered aqueous solution
| Synonym: | Anti-24-dehydro-cholesterol reductase (DCHR24), Anti-Diminuto/Dwarf-1 homolog, Anti-Selective Alzheimer’s disease indicator-1 |
Description
| Immunogen | synthetic peptide corresponding to amino acids 505-516 of human seladin-1, conjugated to KLH. |
| Physical form | Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | SL-14 (monoclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~60 kDa |
| species reactivity | human |
| concentration | ~2 mg/mL |
| application(s) | immunoblotting: 1-2 μg/mL using extracts of HEK-293T cells expressing human seladin-1 |
| immunocytochemistry: suitable | |
| indirect ELISA: suitable | |
| isotype | IgG2b |
| Gene Information | human ... DHCR24(1718) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
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| Array | XP725, Panorama® Antibody Microarray - XPRESS Profiler725 Kit |
